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Last updated: 2017/12/10

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TDG 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00006996-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-TDG full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 45.21
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-TDG antibody (H00006996-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00006996-T01
    TDG transfected lysate.
    Western Blot
    QC Testing of H00006996-T01
    Lane 1: TDG transfected lysate ( 45.21 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 6996
  • Gene Name:
  • TDG
  • Gene Alias:
  • -
  • Gene Description:
  • thymine-DNA glycosylase
  • Gene Summary:
  • The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq
  • Other Designations:
  • G/T mismatch-specific thymine DNA glycosylase
  • Gene Pathway
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