TCP1 (Human) Recombinant Protein (P01)
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More Files
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Specification
Product Description
Human TCP1 full-length ORF ( AAH00665, 1 a.a. - 556 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MEGPLSVFGDRSTGETIRSQNVMAAASIANIVKSSFGPVGLDKMLVDDIGDVTITNDGATILKLLEVEHPAAKVLCELADLQDKEVGDGTTSVVIIAAELLKNADELVKQKIHPTSVISGYRLACKEAVRYINENLIVNTDELGRDCLINAAKTSMSSKIIGINGDFFANMVVDAVLAIKYTDIRGQPRYPVNSVNILKAHGRSQMESMLISGYALNCVVGSQGMPKRIVNAKIACLDFSLQKTKMKLGVQVVITDPEKLDQIRQRESDITKERIQKILATGANVILTTGGIDDMCLKYFVEAGAMAVRRVLKRDLKRIAKASGATILSTLANLEGEETFEAAMLGQAEEVVQERICDDELILIKNTKARTSASIILRGANDFMCDEMERSLHDALCVVKRVLESKSVVPGGGAVEAALSIYLENYATSMGSREQLAIAEFARSLLVIPNTLAVNAAQDSTDLVAKLRAFHNEAQVNPERKNLKWIGLDLSNGKPRDNKQAGVFEPTIVKVKSLKFATEAAITILRIDDLIKLHPESKDDKHGSYEDAVHSGALND
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
87.01
Interspecies Antigen Sequence
Mouse (97); Rat (97)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — TCP1
Entrez GeneID
6950GeneBank Accession#
BC000665Protein Accession#
AAH00665Gene Name
TCP1
Gene Alias
CCT-alpha, CCT1, CCTa, D6S230E, TCP-1-alpha
Gene Description
t-complex 1
Omim ID
186980Gene Ontology
HyperlinkGene Summary
This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
OTTHUMP00000017529|T-complex protein 1|T-complex protein 1, alpha subunit|tailless complex polypeptide 1
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Interactome
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Disease
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Publication Reference
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Serum antibody response to group II chaperonin from Methanobrevibacter oralis and human chaperonin CCT.
Hirai K, Maeda H, Omori K, Yamamoto T, Kokeguchi S, Takashiba S.
Pathogens and Disease 2013 Jun; 68(1):12.
Application:Dot, Human, Serum.
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Serum antibody response to group II chaperonin from Methanobrevibacter oralis and human chaperonin CCT.
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