TCF4 monoclonal antibody (M03), clone 1G4
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant TCF4.
Immunogen
TCF4 (AAH31056.1, 1 a.a. ~ 365 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MHHQQRMAALGTDKELSDLLDFSAMFSPPVSSGKNGPTSLASGHFTGSNVEDRSSSGSWGNGGHPSPSRNYGDGTPYDHTTSRDLGSHDNLSPPFVNSRIQSKTERGSYSSYGRESNLQGCHQQSLLGGDMDMGNPGTLSPTKPGSQYYQYSSNNPRRRPLHSSAMEVQTKKVRKVPPGLPSSVYAPSASTADYNRDSPGYPSSKPATSTFPSSFFMQDGHHSSDPWSSSSGMNQPGYAGMLGNSSHIPQSSSYCSLHPHERLSYPSHSSADINSSLPPMSTFHRSGTNHYSTSSCTPPANGTDSIMANRGSGAAGSSQTGDALGKALASIYSPDHTNNSFSSNPSTPVGSPPSLSAGTAVWSRN
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (98)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (65.89 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged TCF4 is approximately 1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to TCF4 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — TCF4
Entrez GeneID
6925GeneBank Accession#
BC031056.1Protein Accession#
AAH31056.1Gene Name
TCF4
Gene Alias
E2-2, ITF2, MGC149723, MGC149724, PTHS, SEF2, SEF2-1, SEF2-1A, SEF2-1B, bHLHb19
Gene Description
transcription factor 4
Gene Ontology
HyperlinkGene Summary
This gene encodes transcription factor 4, a basic helix-turn-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is expressed predominantly in pre-B-cells, although it is found in other tissues as well. Defects in this gene are a cause of Pitt-Hopkins syndrome. Multiple alternatively spliced transcript variants that encode different proteins have been described. [provided by RefSeq
Other Designations
immunoglobulin transcription factor 2
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Interactome
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Disease
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Publication Reference
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Tcf4 Encodes Correct Brain Development During Embryogenesis.
Simone Mesman, Reinier Bakker, Marten P Smidt.
Molecular and Cellular Neurosciences 2020 Jul; 106:103502.
Application:IF, IHC-Fr, Mouse, Mouse brains, Mouse cortex.
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Tumor xenograft modeling identifies an association between TCF4 loss and breast cancer chemoresistance.
Ruiz de Garibay G, Mateo F, Stradella A, Valdés-Mas R, Palomero L, Serra-Musach J, Puente DA, Díaz-Navarro A, Vargas-Parra G, Tornero E, Morilla I, Farré L, Martinez-Iniesta M, Herranz C, McCormack E, Vidal A, Petit A, Soler T, Lázaro C, Puente XS, Villanueva A, Pujana MA.
Disease Models & Mechanisms 2018 May; 11(5):dmm032292.
Application:IHC-P, WB-Tr, Human, Mouse, CAL-51, MDA-MB-436 cells, Mouse tumors.
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A PGC1α-mediated transcriptional axis suppresses melanoma metastasis.
Luo C, Lim JH, Lee Y, Granter SR, Thomas A, Vazquez F, Widlund HR, Puigserver P.
Nature 2016 Sep; 537(7620):422.
Application:WB, Human, A375P cells.
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Knockdown of Human TCF4 Affects Multiple Signaling Pathways Involved in Cell Survival, Epithelial to Mesenchymal Transition and Neuronal Differentiation.
Forrest MP, Waite AJ, Martin-Rendon E, Blake DJ.
PLoS One 2013 Aug; 8(8):e73169.
Application:WB-Tr, Human, SH-SY5Y cells.
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Inhibition of endothelial cell activation by bHLH protein E2-2 and its impairment of angiogenesis.
Tanaka A, Itoh F, Nishiyama K, Takezawa T, Kurihara H, Itoh S, Kato M.
Blood 2010 May; 115(20):4138.
Application:IF, IP, WB-Tr, Monkey, Mouse, COS-7, Mouse embryonic endothelial cells.
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Tcf4 Encodes Correct Brain Development During Embryogenesis.
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