TCEB3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-TCEB3 full-length
Host
Human
Theoretical MW (kDa)
85.03
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TCEB3 antibody (H00006924-B01) by Western Blots.
SDS-PAGE Gel
TCEB3 transfected lysate.
Western Blot
Lane 1: TCEB3 transfected lysate ( 85.03 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — TCEB3
Entrez GeneID
6924GeneBank Accession#
NM_003198.1Protein Accession#
NP_003189.1Gene Name
TCEB3
Gene Alias
FLJ38760, FLJ42849, SIII, TCEB3A
Gene Description
transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A)
Omim ID
600786Gene Ontology
HyperlinkGene Summary
This gene encodes the protein elongin A, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. [provided by RefSeq
Other Designations
OTTHUMP00000002990|elongin A|transcription elongation factor B (SIII), polypeptide 3 (110kD)|transcription elongation factor B alpha subunit
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