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TAF12 rabbit monoclonal antibody

  • Catalog # : H00006883-K
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against a human TAF12 peptide using ARM Technology.
  • Immunogen:
  • A synthetic peptide of human TAF12 is used for rabbit immunization.
    Customer or Abnova will decide on the preferred peptide sequence.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Purification:
  • Protein A
  • Isotype:
  • IgG
  • Quality Control Testing:
  • Antibody reactive against human TAF12 peptide by ELISA and mammalian transfected lysate by Western Blot.
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Note:
  • 1. Customer may provide cell or tissue lysate for antibody screening.
    2. Rabbit monoclonal antibody generated by ARM technology is amenable to antibody engineering including F(ab)2, IgG, scFv and different Fc and non-Fc conjugates per customer request.
  • Library Construction:
  • Non-fusion antibody library from rabbit spleen (ARM Technology).
  • Expression:
  • Overexpression vector and transfection into 293H cell line.
  • Deliverable:
  • Up to three rabbit IgG clones of 1 mg each will be delivered to customer.
  • Applications
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 6883
  • GeneBank Accession#:
  • TAF12
  • Gene Name:
  • TAF12
  • Gene Alias:
  • TAF2J,TAFII20
  • Gene Description:
  • TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa
  • Gene Summary:
  • Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000003785,OTTHUMP00000003786,TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20 kD,TATA box binding protein (TBP)-associated factor, RNA polymerase II, J, 20kD,transcription initiation factor TFIID 20/15 kDa subunits
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