TAF10 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-TAF10 full-length
Host
Human
Theoretical MW (kDa)
24.09
Interspecies Antigen Sequence
Mouse (92); Rat (93)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TAF10 antibody (H00006881-B01) by Western Blots.
SDS-PAGE Gel
TAF10 transfected lysate.
Western Blot
Lane 1: TAF10 transfected lysate ( 24.09 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — TAF10
Entrez GeneID
6881GeneBank Accession#
NM_006284.2Protein Accession#
-Gene Name
TAF10
Gene Alias
TAF2A, TAF2H, TAFII30
Gene Description
TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 30kDa
Omim ID
600475Gene Ontology
HyperlinkGene Summary
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the small subunits of TFIID that is associated with a subset of TFIID complexes. Studies with human and mammalian cells have shown that this subunit is required for transcriptional activation by the estrogen receptor, for progression through the cell cycle, and may also be required for certain cellular differentiation programs. [provided by RefSeq
Other Designations
TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 30 kD|TATA box binding protein (TBP)-associated factor, RNA polymerase II, H, 30kD|TBP-related factor 10|transcription initiation factor TFIID 30 kD subunit
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Interactome
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Pathway
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