TAF10 (Human) IP-WB Antibody Pair
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Specification
Product Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity
Human
Interspecies Antigen Sequence
Mouse (91); Rat (93)
Quality Control Testing
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of TAF10 transfected lysate using rabbit polyclonal anti-TAF10 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse polyclonal anti-TAF10.
Supplied Product
Antibody pair set content:
1. Antibody pair for IP: rabbit polyclonal anti-TAF10 (300 ul)
2. Antibody pair for WB: mouse polyclonal anti-TAF10 (50 ul)
Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
Immunoprecipitation-Western Blot
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Gene Info — TAF10
Entrez GeneID
6881Gene Name
TAF10
Gene Alias
TAF2A, TAF2H, TAFII30
Gene Description
TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 30kDa
Omim ID
600475Gene Ontology
HyperlinkGene Summary
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the small subunits of TFIID that is associated with a subset of TFIID complexes. Studies with human and mammalian cells have shown that this subunit is required for transcriptional activation by the estrogen receptor, for progression through the cell cycle, and may also be required for certain cellular differentiation programs. [provided by RefSeq
Other Designations
TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 30 kD|TATA box binding protein (TBP)-associated factor, RNA polymerase II, H, 30kD|TBP-related factor 10|transcription initiation factor TFIID 30 kD subunit
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Interactome
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Pathway
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