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TAF6 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00006878-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-TAF6 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 74.58
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-TAF6 antibody (H00006878-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00006878-T01
    TAF6 transfected lysate.
    Western Blot
    QC Testing of H00006878-T01
    Lane 1: TAF6 transfected lysate ( 74.58 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 6878
  • Protein Accession#:
  • -
  • Gene Name:
  • TAF6
  • Gene Alias:
  • DKFZp781E21155,MGC:8964,TAF2E,TAFII70,TAFII80,TAFII85
  • Gene Description:
  • TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80kDa
  • Gene Summary:
  • Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. Four isoforms have been identified but complete transcripts have been determined for only three isoforms. One of the isoforms has been shown to preclude binding of one of the other TFIID subunits, thereby reducing transcription and initiating signals that trigger apoptosis. [provided by RefSeq
  • Other Designations:
  • TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80 kD,TATA box binding protein (TBP)-associated factor, RNA polymerase II, E, 70/85kD,TBP-associated factor 6,transcription initiation factor TFIID 70 kD subunit
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