SURF1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
SURF1 transfected lysate.
Western Blot
Lane 1: SURF1 transfected lysate ( 33.11 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SURF1 full-length
Host
Human
Theoretical MW (kDa)
33.11
Interspecies Antigen Sequence
Mouse (78); Rat (76)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SURF1 antibody (H00006834-B01) by Western Blots.
SDS-PAGE Gel
SURF1 transfected lysate.
Western Blot
Lane 1: SURF1 transfected lysate ( 33.11 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SURF1
Entrez GeneID
6834GeneBank Accession#
NM_003172.2Protein Accession#
NP_003163.1Gene Name
SURF1
Gene Alias
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Gene Description
surfeit 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein localized to the inner mitochondrial membrane and thought to be involved in the biogenesis of the cytochrome c oxidase complex. The protein is a member of the SURF1 family, which includes the related yeast protein SHY1 and rickettsial protein RP733. The gene is located in the surfeit gene cluster, a group of very tightly linked genes that do not share sequence similarity, where it shares a bidirectional promoter with SURF2 on the opposite strand. Defects in this gene are a cause of Leigh syndrome, a severe neurological disorder that is commonly associated with systemic cytochrome c oxidase deficiency. [provided by RefSeq
Other Designations
OTTHUMP00000022473|surfeit locus protein 1
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Interactome
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Disease
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