SUOX monoclonal antibody (M01), clone 1H1
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More Files
- More Functions
Western Blot (Cell lysate)
SUOX monoclonal antibody (M01), clone 1H1 Western Blot analysis of SUOX expression in HepG2 ( Cat # L019V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of SUOX expression in transfected 293T cell line by SUOX monoclonal antibody (M01), clone 1H1.
Lane 1: SUOX transfected lysate(53.9 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SUOX is 0.03 ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of SUOX over-expressed 293 cell line, cotransfected with SUOX Validated Chimera RNAi ( Cat # H00006821-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with SUOX monoclonal antibody (M01), clone 1H1 (Cat # H00006821-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot detection against Immunogen (79.42 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant SUOX.
Immunogen
SUOX (AAH65193, 1 a.a. ~ 488 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MGTLLGLGAVLAYQDHRCRAAQESTHIYTKEEVSSHTSPETGIWVTLGSEVFDVTEFVDLHPGGPSKLMLAAGGPLEPFWALYAVHNQSHVRELLAQYKIGELNPEDKVAPTVETSDPYADDPVRHPALKVNSQRPFNAEPPPELLTENYITPNPIFFTRNHLPVPNLDPDTYRLHVVGAPGGQSLSLSLDDLHNFPRYEITVTLQCAGNRRSEMTQVKEVKGLEWRTGAISTARWAGARLCDVLAQAGHQLCETEAHVCFEGLDSDPTGTAYGASIPLARAMDPEAEVLLAYEMNGQPLPRDHGFPVRVVVPGVVGARHVKWLGRVSVQPEESYSHWQRRDYKGFSPSVDWETVDFDSAPSIQELPVQSAITEPRDGETVESGEVTIKGYAWSGGGRAVIRVDVSLDGGLTWQVAKLDGEEQRPRKAWAWRLWQLKAPVPAGQKELNIVCKAVDDGYNVQPDTVAPIWNLRGVLSNAWHRVHVYVSP
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (88); Rat (88)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (79.42 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
SUOX monoclonal antibody (M01), clone 1H1 Western Blot analysis of SUOX expression in HepG2 ( Cat # L019V1 ).Western Blot (Transfected lysate)
Western Blot analysis of SUOX expression in transfected 293T cell line by SUOX monoclonal antibody (M01), clone 1H1.
Lane 1: SUOX transfected lysate(53.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SUOX is 0.03 ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of SUOX over-expressed 293 cell line, cotransfected with SUOX Validated Chimera RNAi ( Cat # H00006821-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with SUOX monoclonal antibody (M01), clone 1H1 (Cat # H00006821-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — SUOX
Entrez GeneID
6821GeneBank Accession#
BC065193Protein Accession#
AAH65193Gene Name
SUOX
Gene Alias
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Gene Description
sulfite oxidase
Gene Ontology
HyperlinkGene Summary
Sulfite oxidase is a homodimeric protein localized to the intermembrane space of mitochondria. Each subunit contains a heme domain and a molybdopterin-binding domain. The enzyme catalyzes the oxidation of sulfite to sulfate, the final reaction in the oxidative degradation of the sulfur amino acids cysteine and methionine. Sulfite oxidase deficiency results in neurological abnormalities which are often fatal at an early age. Alternative splicing results in multiple transcript variants encoding identical proteins. [provided by RefSeq
Other Designations
OTTHUMP00000158619
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Interactome
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Pathway
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Disease
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Publication Reference
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Demonstration of autoantibodies to recombinant human sulphite oxidase in patients with chronic liver disorders and analysis of their clinical relevance.
Preuss B, Berg C, Altenberend F, Gregor M, Stevanovic S, Klein R.
Clinical and Experimental Immunology 2007 Aug; 150(2):312.
Application：WB, Recombinant protein.
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Demonstration of autoantibodies to recombinant human sulphite oxidase in patients with chronic liver disorders and analysis of their clinical relevance.
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