STK3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
STK3 transfected lysate.
Western Blot
Lane 1: STK3 transfected lysate ( 56.30 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-STK3 full-length
Host
Human
Theoretical MW (kDa)
56.3
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-STK3 antibody (H00006788-D01P) by Western Blots.
SDS-PAGE Gel
STK3 transfected lysate.
Western Blot
Lane 1: STK3 transfected lysate ( 56.30 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — STK3
Entrez GeneID
6788GeneBank Accession#
NM_006281.2Protein Accession#
NP_006272.2Gene Name
STK3
Gene Alias
FLJ90748, KRS1, MST2
Gene Description
serine/threonine kinase 3 (STE20 homolog, yeast)
Omim ID
605030Gene Ontology
HyperlinkGene Summary
Protein kinase activation is a frequent response of cells to treatment with growth factors, chemicals, heat shock, or apoptosis-inducing agents. This protein kinase activation presumably allows cells to resist unfavorable environmental conditions. The yeast 'sterile 20' (Ste20) kinase acts upstream of the mitogen-activated protein kinase (MAPK) cascade that is activated under a variety of stress conditions. MST2 was identified as a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand (MIM 134638) (Taylor et al., 1996 [PubMed 8816758]; Lee et al., 2001 [PubMed 11278283]).[supplied by OMIM
Other Designations
serine/threonine kinase 3 (Ste20, yeast homolog)
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