STAT4 monoclonal antibody (M01), clone 1C2-1C12
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More Files
- More Functions
Western Blot (Tissue lysate)
STAT4 monoclonal antibody (M01), clone 1C2-1C12. Western Blot analysis of STAT4 expression in human liver.
Western Blot (Cell lysate)
STAT4 monoclonal antibody (M01), clone 1C2-1C12 Western Blot analysis of STAT4 expression in A-549 ( Cat # L025V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of STAT4 expression in transfected 293T cell line by STAT4 monoclonal antibody (M01), clone 1C2-1C12.
Lane 1: STAT4 transfected lysate(85.9 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to STAT4 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue. [antibody concentration 1 ~ 10 ug/ml]
Immunoprecipitation
Immunoprecipitation of STAT4 transfected lysate using anti-STAT4 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with STAT4 MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged STAT4 is approximately 0.3ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to STAT4 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (108.02 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant STAT4.
Immunogen
STAT4 (AAH31212, 1 a.a. ~ 748 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSQWNQVQQLEIKFLEQVDQFYDDNFPMEIRHLLAQWIENQDWEAASNNETMATILLQNLLIQLDEQLGRVSKEKNLLLIHNLKRIRKVLQGKFHGNPMHVAVVISNCLREERRILAAANMPVQGPLEKSLQSSSVSERQRNVEHKVAAIKNSVQMTEQDTKYLEDLQDEFDYRYKTIQTMDQSDKNSAMVNQEVLTLQEMLNSLDFKRKEALSKMTQIIHETDLLMNTMLIEELQDWKRRQQIACIGGPLHNGLDQLQNCFTLLAESLFQLRRQLEKLEEQSTKMTYEGDPIPMQRTHMLERVTFLIYNLFKNSFVVERQPCMPTHPQRPLVLKTLIQFTVKLRLLIKLPELNYQVKVKASIDKNVSTLSNRRFVLCGTNVKAMSIEESSNGSLSVEFRHLQPKEMKSSAGGKGNEGCHMVTEELHSITFETQICLYGLTIDLETSSLPVVMISNVSQLPNAWASIIWYNVSTNDSQNLVFFNNPPPATLSQLLEVMSWQFSSYVGRGLNSDQLHMLAEKLTVQSSYSDGHLTWAKFCKEHLPGKSFTFWTWLEAILDLIKKHILPLWIDGYVMGFVSKEKERLLLKDKMPGTFLLRFSESHLGGITFTWVDHSESGEVRFHSVEPYNKGRLSALPFADILRDYKVIMAENIPENPLKYLYPDIPKDKAFGKHYSSQPCEVSRPTERGDKGYVPSVFIPISTIRSDSTEPHSPSDLLPMSPSVYAVLRENLSPTTIETAMKSPYSAE
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (108.02 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
STAT4 monoclonal antibody (M01), clone 1C2-1C12. Western Blot analysis of STAT4 expression in human liver.Western Blot (Cell lysate)
STAT4 monoclonal antibody (M01), clone 1C2-1C12 Western Blot analysis of STAT4 expression in A-549 ( Cat # L025V1 ).Western Blot (Transfected lysate)
Western Blot analysis of STAT4 expression in transfected 293T cell line by STAT4 monoclonal antibody (M01), clone 1C2-1C12.
Lane 1: STAT4 transfected lysate(85.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to STAT4 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue. [antibody concentration 1 ~ 10 ug/ml]Immunoprecipitation
Immunoprecipitation of STAT4 transfected lysate using anti-STAT4 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with STAT4 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged STAT4 is approximately 0.3ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to STAT4 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — STAT4
Entrez GeneID
6775GeneBank Accession#
BC031212Protein Accession#
AAH31212Gene Name
STAT4
Gene Alias
SLEB11
Gene Description
signal transducer and activator of transcription 4
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. [provided by RefSeq
Other Designations
OTTHUMP00000163553
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Interactome
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Pathway
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Disease
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