STAR monoclonal antibody (M01J), clone 5F9
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant STAR.
This product is belong to Cell Culture Grade Antibody (CX Grade).Immunogen
STAR (AAH10550, 81 a.a. ~ 180 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
EAMQKALGILSNQEGWKKESQQDNGDKVMSKVVPDVGKVFRLEVVVDQPMERLYEELVERMEAMGEWNPNVKEIKVLQKIGKDTFITHELAAEAAGNLVG
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (90); Rat (88)
Preparation Method
Cell Culture Production
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of STAR expression in transfected 293T cell line by STAR monoclonal antibody (M01J), clone 5F9.
Lane 1: STAR transfected lysate (Predicted MW: 31.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged STAR is 0.03 ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of STAR over-expressed 293 cell line, cotransfected with STAR Validated Chimera RNAi ( Cat # H00006770-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with STAR monoclonal antibody (M01), clone 5F9 (Cat # H00006770-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — STAR
Entrez GeneID
6770GeneBank Accession#
BC010550Protein Accession#
AAH10550Gene Name
STAR
Gene Alias
STARD1
Gene Description
steroidogenic acute regulatory protein
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13. [provided by RefSeq
Other Designations
START domain containing 1|StAR-related lipid transfer (START) domain containing 1|cholesterol trafficker|mitochondrial steroid acute regulatory protein|steroid acute regulatory protein|steroidogenic acute regulator
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Interactome
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Disease
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