AKR1D1 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-AKR1D1 full-length
Host
Human
Theoretical MW (kDa)
35.97
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-AKR1D1 antibody (H00006718-B01) by Western Blots.
SDS-PAGE Gel
AKR1D1 transfected lysate.
Western Blot
Lane 1: AKR1D1 transfected lysate ( 35.97 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — AKR1D1
Entrez GeneID
6718GeneBank Accession#
NM_005989.2Protein Accession#
NP_005980.1Gene Name
AKR1D1
Gene Alias
3o5bred, SRD5B1
Gene Description
aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase)
Omim ID
604741Gene Ontology
HyperlinkGene Summary
The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction. [provided by RefSeq
Other Designations
aldo-keto reductase family 1, member D1|steroid 5-beta-reductase|steroid-5-beta-reductase, beta polypeptide 1 (3-oxo-5 beta-steroid delta 4-dehydrogenase beta 1)
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Interactome
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Pathway
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Disease
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