Products

Western Blot (Transfected lysate)

Western Blot analysis of AKR1D1 expression in transfected 293T cell line by AKR1D1 monoclonal antibody (M03), clone 1C2.

Lane 1: AKR1D1 transfected lysate(37.4 KDa).
Lane 2: Non-transfected lysate.

Western Blot (Recombinant protein)

Sandwich ELISA (Recombinant protein)

Detection limit for recombinant GST tagged AKR1D1 is approximately 0.3ng/ml as a capture antibody.

ELISA

RNAi Knockdown (Antibody validated)

Western blot analysis of AKR1D1 over-expressed 293 cell line, cotransfected with AKR1D1 Validated Chimera RNAi ( Cat # H00006718-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with AKR1D1 monoclonal antibody (M03), clone 1C2 (Cat # H00006718-M03 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Entrez GeneID

6718

GeneBank Accession#

NM_005989

Protein Accession#

NP_005980

Gene Name

AKR1D1

Gene Alias

3o5bred, SRD5B1

Gene Description

aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase)

Omim ID

604741

Gene Ontology

Hyperlink

Gene Summary

The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction. [provided by RefSeq

Other Designations

aldo-keto reductase family 1, member D1|steroid 5-beta-reductase|steroid-5-beta-reductase, beta polypeptide 1 (3-oxo-5 beta-steroid delta 4-dehydrogenase beta 1)