SRC 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
SRC transfected lysate.
Western Blot
Lane 1: SRC transfected lysate ( 59.07 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SRC full-length
Host
Human
Theoretical MW (kDa)
59.07
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SRC antibody (H00006714-B01) by Western Blots.
SDS-PAGE Gel
SRC transfected lysate.
Western Blot
Lane 1: SRC transfected lysate ( 59.07 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SRC
Entrez GeneID
6714GeneBank Accession#
NM_005417.3Protein Accession#
NP_005408.1Gene Name
SRC
Gene Alias
ASV, SRC1, c-SRC, p60-Src
Gene Description
v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
Omim ID
190090Gene Ontology
HyperlinkGene Summary
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000174476|OTTHUMP00000174477|proto-oncogene tyrosine-protein kinase SRC|protooncogene SRC, Rous sarcoma|tyrosine kinase pp60c-src|tyrosine-protein kinase SRC-1
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Interactome
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Pathway
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Disease
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