SPI1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
SPI1 transfected lysate.
Western Blot
Lane 1: SPI1 transfected lysate ( 30.40 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SPI1 full-length
Host
Human
Theoretical MW (kDa)
30.4
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SPI1 antibody (H00006688-D01P) by Western Blots.
SDS-PAGE Gel
SPI1 transfected lysate.
Western Blot
Lane 1: SPI1 transfected lysate ( 30.40 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SPI1
Entrez GeneID
6688GeneBank Accession#
NM_003120.1Protein Accession#
ENSP00000227163Gene Name
SPI1
Gene Alias
OF, PU.1, SFPI1, SPI-1, SPI-A
Gene Description
spleen focus forming virus (SFFV) proviral integration oncogene spi1
Omim ID
165170Gene Ontology
HyperlinkGene Summary
This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
31 kDa transforming protein|SPI-1 proto-oncogene|hematopoietic transcription factor PU.1
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Interactome
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Pathway
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Disease
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