SPI1 (Human) Recombinant Protein (P01)
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More Files
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Specification
Product Description
Human SPI1 full-length ORF ( ENSP00000227163, 1 a.a. - 264 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MEGFPLVPPPSEDLVPYDTDLYQRQTHEYYPYLSSDGESHSDHYWDFHPHHVHSEFESFAENNFTELQSVQPPQLQQLYRHMELEQMHVLDTPMVPPHPSLGHQVSYLPRMCLQYPSLSPAQPSSDEEEGERQSPPLEVSDGEADGLEPGPGLLPGETGSKKKIRLYQFLLDLLRSGDMKDSIWWVDKDKGTFQFSSKHKEALAHRWGIQKGNRKKMTYQKMARALRNYGKTGEVKKVKKKLTYQFSGEVLGRGGLAERRHPPH
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
56.8
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — SPI1
Entrez GeneID
6688GeneBank Accession#
ENST00000227163Protein Accession#
ENSP00000227163Gene Name
SPI1
Gene Alias
OF, PU.1, SFPI1, SPI-1, SPI-A
Gene Description
spleen focus forming virus (SFFV) proviral integration oncogene spi1
Omim ID
165170Gene Ontology
HyperlinkGene Summary
This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
31 kDa transforming protein|SPI-1 proto-oncogene|hematopoietic transcription factor PU.1
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Interactome
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Pathway
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Disease
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