SPG7 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
SPG7 transfected lysate.
Western Blot
Lane 1: SPG7 transfected lysate ( 87.56 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SPG7 full-length
Host
Human
Theoretical MW (kDa)
87.56
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SPG7 antibody (H00006687-B01) by Western Blots.
SDS-PAGE Gel
SPG7 transfected lysate.
Western Blot
Lane 1: SPG7 transfected lysate ( 87.56 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SPG7
Entrez GeneID
6687GeneBank Accession#
NM_003119.2Protein Accession#
NP_003110.1Gene Name
SPG7
Gene Alias
CAR, CMAR, FLJ37308, MGC126331, MGC126332, PGN, SPG5C
Gene Description
spastic paraplegia 7 (pure and complicated autosomal recessive)
Gene Ontology
HyperlinkGene Summary
This gene encodes a nuclear-encoded mitochondrial metalloprotease protein that is a member of the AAA (ATPases associated with a variety of cellular activities) protein family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Two transcript variants encoding distinct isoforms have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 7. [provided by RefSeq
Other Designations
cell adhesion regulator|cell matrix adhesion regulator|paraplegin, isoform 1|spastic paraplegia 7
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Interactome
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Disease
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