SPARC (Human) Recombinant Protein (P01)
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More Files
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Specification
Product Description
Human SPARC full-length ORF ( AAH04974.1, 1 a.a. - 303 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MRAWIFFLLCLAGRALAAPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFDDGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCPAPIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCKYIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTEKQKLRVKKIHENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQKDIDKDLVI
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
59.07
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — SPARC
Entrez GeneID
6678GeneBank Accession#
BC004974Protein Accession#
AAH04974.1Gene Name
SPARC
Gene Alias
ON
Gene Description
secreted protein, acidic, cysteine-rich (osteonectin)
Omim ID
182120Gene Ontology
HyperlinkGene Summary
Secreted protein acidic and rich in cysteine/osteonectin/BM40, or SPARC, is a matrix-associated protein that elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (ECM) (Bradshaw et al., 2003 [PubMed 12721366]).[supplied by OMIM
Other Designations
cysteine-rich protein|osteonectin|secreted protein, acidic, cysteine-rich
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Interactome
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Disease
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