SPAG4 monoclonal antibody (M04), clone 3C8
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Specifications
Product Description
Mouse monoclonal antibody raised against a partial recombinant SPAG4.
Immunogen
SPAG4 (NP_003107, 221 a.a. ~ 330 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
AELDKLHKEVSTVRAANSERVAKLVFQRLNEDFVRKPDYALSSVGASIDLQKTSHDYADRNTAYFWNRFSFWNYARPPTVILEPHVFPGNCWAFEGDQGQVVIQLPGRVQ
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (88); Rat (88)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.84 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SPAG4 is 1 ng/ml as a capture antibody.ELISA
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Gene Info — SPAG4
Entrez GeneID
6676GeneBank Accession#
NM_003116Protein Accession#
NP_003107Gene Name
SPAG4
Gene Alias
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Gene Description
sperm associated antigen 4
Omim ID
603038Gene Ontology
HyperlinkGene Summary
The mammalian sperm flagellum contains two cytoskeletal structures associated with the axoneme: the outer dense fibers surrounding the axoneme in the midpiece and principal piece and the fibrous sheath surrounding the outer dense fibers in the principal piece of the tail. Defects in these structures are associated with abnormal tail morphology, reduced sperm motility, and infertility. In the rat, the protein encoded by this gene associates with an outer dense fiber protein via a leucine zipper motif and localizes to the microtubules of the manchette and axoneme during sperm tail development. [provided by RefSeq
Other Designations
OTTHUMP00000030768|acrosomal protein ACR55|outer dense fiber-associated protein SPAG4|sperm tail protein
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