SP100 MaxPab mouse polyclonal antibody (B01)
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of SP100 expression in transfected 293T cell line (H00006672-T01) by SP100 MaxPab polyclonal antibody.
Lane 1: SP100 transfected lysate(52.8 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of purified MaxPab antibody to SP100 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml]
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human SP100 protein.
Immunogen
SP100 (AAH11562.1, 1 a.a. ~ 480 a.a) full-length human protein.
Sequence
MAGGGGDLSTRRLNECISPVANEMNHLPAHSHDLQRMFTEDQGVDDRLLYDIVFKHFKRNKVEISNAIKKTFPFLEGLRDRDLITNKMFEDSQDSCRNLVPVQRVVYNVLSELEKTFNLPVLEALFSDVNMQEYPDLIHIYKGFENVIHDKLPLQESEEEEREERSGLQLSLEQGTGENSFRSLTWPPSGSPSHAGTTPPENGLSEHPCETEQINAKRKDTTSDKDDSLGSQQTNEQCAQKAEPTESCEQIAVQVNNGDAGREMPCPLPCDEESPEAELHNHGIQINSCSVRLVDIKKEKPFSNSKVECQAQARTHHNQASDIIVISSEDSEGSTDVDEPLEVFISAPRSEPVINNDNPLESNDEKEGQEATCSRPQIVPEPMDFRKLSTFRESFKKRVIGQDHDFSESSEEEAPAEASSGALRSKHGEKAPMTSRSTSTWRIPSRKRRFSSSDFSDLSNGEELQETCSSSLRRGSGKED
Host
Mouse
Reactivity
Human
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
No additive
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Note
For IHC and IF applications, antibody purification with Protein A will be needed prior to use.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of SP100 expression in transfected 293T cell line (H00006672-T01) by SP100 MaxPab polyclonal antibody.
Lane 1: SP100 transfected lysate(52.8 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of purified MaxPab antibody to SP100 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml] -
Gene Info — SP100
Entrez GeneID
6672GeneBank Accession#
BC011562.1Protein Accession#
AAH11562.1Gene Name
SP100
Gene Alias
DKFZp686E07254, FLJ00340, FLJ34579
Gene Description
SP100 nuclear antigen
Omim ID
604585Gene Ontology
HyperlinkOther Designations
SP100-HMG nuclear autoantigen|nuclear antigen Sp100
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Interactome
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Disease
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Publication Reference
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Contribution of the Major ND10 Proteins PML, hDaxx and Sp100 to the Regulation of Human Cytomegalovirus Latency and Lytic Replication in the Monocytic Cell Line THP-1.
Wagenknecht N, Reuter N, Scherer M, Reichel A, Muller R, Stamminger T.
Viruses 2015 Jun; 7(6):2884.
Application：IF, WB, WB-Tr, Human, THP-1 cells.
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Kaposi's sarcoma associated herpesvirus tegument protein ORF75 is essential for viral lytic replication and plays a critical role in the antagonization of ND10-instituted intrinsic immunity.
Full F, Jungnickl D, Reuter N, Bogner E, Brulois K, Scholz B, Sturzl M, Myoung J, Jung JU, Stamminger T, Ensser A.
PLoS Pathogens 2014 Jan; 10(1):e1003863.
Application：IF, WB-Tr, Human, iSLK, 293T cells.
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Contribution of the Major ND10 Proteins PML, hDaxx and Sp100 to the Regulation of Human Cytomegalovirus Latency and Lytic Replication in the Monocytic Cell Line THP-1.
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