SP1 monoclonal antibody (M05), clone 4B11
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant SP1.
Immunogen
SP1 (NP_612482, 522 a.a. ~ 618 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
QLSSMPGLQTINLSALGTSGIQVHPIQGLPLAIANAPGDHGAQLGLHGAGGDGIHDDTAGGEEGENSPDAQPQAGRRTRREACTCPYCKDSEGRGSG*
Host
Mouse
Reactivity
Human, Mouse
Interspecies Antigen Sequence
Mouse (89); Rat (93)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.78 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
SP1 monoclonal antibody (M05), clone 4B11. Western Blot analysis of SP1 expression in Raw 264.7(Cat # L024V1 ).Western Blot (Cell lysate)
SP1 monoclonal antibody (M05), clone 4B11 Western Blot analysis of SP1 expression in HeLa ( Cat # L013V1 ).Western Blot (Cell lysate)
SP1 monoclonal antibody (M05), clone 4B11. Western Blot analysis of SP1 expression in NIH/3T3(Cat # L018V1 ).Western Blot (Transfected lysate)
Western Blot analysis of SP1 expression in transfected 293T cell line by SP1 monoclonal antibody (M05), clone 4B11.
Lane 1: SP1 transfected lysate(80.693 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SP1 is approximately 10ng/ml as a capture antibody.ELISA
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Gene Info — SP1
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Interactome
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Pathway
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Disease
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