SOD1 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SOD1 full-length
Host
Human
Theoretical MW (kDa)
15.9
Interspecies Antigen Sequence
Mouse (84); Rat (83)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SOD1 antibody (H00006647-B01P) by Western Blots.
SDS-PAGE Gel
SOD1 transfected lysate.
Western Blot
Lane 1: SOD1 transfected lysate ( 15.90 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SOD1
Entrez GeneID
6647GeneBank Accession#
NM_000454.4Protein Accession#
NP_000445.1Gene Name
SOD1
Gene Alias
ALS, ALS1, IPOA, SOD, homodimer
Gene Description
superoxide dismutase 1, soluble
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq
Other Designations
Cu /Zn superoxide dismutase|Cu/Zn superoxide dismutase|SOD, soluble|indophenoloxidase A|superoxide dismutase, cystolic
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Interactome
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Disease
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