SOAT1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
SOAT1 transfected lysate.
Western Blot
Lane 1: SOAT1 transfected lysate ( 60.61 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SOAT1 full-length
Host
Human
Theoretical MW (kDa)
60.61
Interspecies Antigen Sequence
Mouse (86); Rat (85)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SOAT1 antibody (H00006646-B01) by Western Blots.
SDS-PAGE Gel
SOAT1 transfected lysate.
Western Blot
Lane 1: SOAT1 transfected lysate ( 60.61 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SOAT1
Entrez GeneID
6646GeneBank Accession#
NM_003101.4Protein Accession#
NP_003092.4Gene Name
SOAT1
Gene Alias
ACACT, ACAT, ACAT1, RP11-215I23.2, SOAT, STAT
Gene Description
sterol O-acyltransferase 1
Omim ID
102642Gene Ontology
HyperlinkGene Summary
Acyl-coenzyme A:cholesterol acyltransferase (ACACT; EC 2.3.1.26) is an intracellular protein located in the endoplasmic reticulum that forms cholesterol esters from cholesterol. Accumulation of cholesterol esters as cytoplasmic lipid droplets within macrophages and smooth muscle cells is a characteristic feature of the early stages of atherosclerotic plaques (Cadigan et al., 1988 [PubMed 3335499]).[supplied by OMIM
Other Designations
acyl-Coenzyme A: cholesterol acyltransferase|sterol O-acyltransferase (acyl-Coenzyme A: cholesterol acyltransferase) 1
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Interactome
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Pathway
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Disease
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