SLC1A3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
SLC1A3 transfected lysate.
Western Blot
Lane 1: SLC1A3 transfected lysate ( 59.50 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SLC1A3 full-length
Host
Human
Theoretical MW (kDa)
59.5
Interspecies Antigen Sequence
Mouse (96); Rat (97)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SLC1A3 antibody (H00006507-D01P) by Western Blots.
SDS-PAGE Gel
SLC1A3 transfected lysate.
Western Blot
Lane 1: SLC1A3 transfected lysate ( 59.50 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SLC1A3
Entrez GeneID
6507GeneBank Accession#
NM_004172.3Protein Accession#
NP_004163.2Gene Name
SLC1A3
Gene Alias
EA6, EAAT1, FLJ25094, GLAST, GLAST1
Gene Description
solute carrier family 1 (glial high affinity glutamate transporter), member 3
Omim ID
600111Gene Ontology
HyperlinkGene Summary
Glutamate and aspartate are excitatory neurotransmitters that have been implicated in a number of pathologic states of the nervous system. Accumulation of extracellular excitatory amino acids can be cytotoxic and may also lower the seizure threshold in epilepsy. EAAT1 (SLC1A3) is a member of a family of high-affinity sodium-dependent transporter molecules that regulate neurotransmitter concentrations at the excitatory glutamatergic synapses of the mammalian central nervous system (Kirschner et al., 1994 [PubMed 8001975]).[supplied by OMIM
Other Designations
glutamate transporter variant EAAT1ex9skip
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Interactome
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Disease
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