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SHMT1 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00006470-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-SHMT1 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 53.24
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-SHMT1 antibody (H00006470-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00006470-T01
    SHMT1 transfected lysate.
    Western Blot
    QC Testing of H00006470-T01
    Lane 1: SHMT1 transfected lysate ( 53.24 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 6470
  • Gene Name:
  • SHMT1
  • Gene Alias:
  • CSHMT,MGC15229,MGC24556,SHMT
  • Gene Description:
  • serine hydroxymethyltransferase 1 (soluble)
  • Gene Summary:
  • This gene encodes the cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative splicing of this gene results in 2 transcript variants encoding 2 different isoforms. Additional transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq
  • Other Designations:
  • 14 kDa protein,cytoplasmic serine hydroxymethyltransferase
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