This antibody pair set comes with matched antibody pair to detect and quantify protein level of human SHMT1.
Quality Control Testing:
Standard curve using SHMT1 293T overexpression lysate (non-denatured) as an analyte.
Sandwich ELISA detection sensitivity ranging from approximately 729x to 3x dilution of the SHMT1 293T overexpression lysate (non-denatured).
Antibody pair set content: 1. Capture antibody: mouse monoclonal anti-SHMT1 (100 ug) 2. Detection antibody: rabbit purified polyclonal anti-SHMT1 (50 ug) *Reagents are sufficient for at least 3-5 x 96 well plates using recommended protocols.
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
This gene encodes the cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative splicing of this gene results in 2 transcript variants encoding 2 different isoforms. Additional transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq