SGSH 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SGSH full-length
Host
Human
Theoretical MW (kDa)
55.33
Interspecies Antigen Sequence
Mouse (89)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SGSH antibody (H00006448-B01) by Western Blots.
SDS-PAGE Gel
SGSH transfected lysate.
Western Blot
Lane 1: SGSH transfected lysate ( 55.33 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SGSH
Entrez GeneID
6448GeneBank Accession#
NM_000199.2Protein Accession#
NP_000190.1Gene Name
SGSH
Gene Alias
HSS, MPS3A, SFMD
Gene Description
N-sulfoglucosamine sulfohydrolase
Gene Ontology
HyperlinkGene Summary
This gene encodes one of several enzymes involved in the lysosomal degradation of heparan sulfate. Mutations in this gene are associated with Sanfilippo syndrome A, one type of the lysosomal storage disease mucopolysaccaridosis III, which results from impaired degradation of heparan sulfate. Transcripts of varying sizes have been reported but their biological validity has not been determined. [provided by RefSeq
Other Designations
heparan sulfate sulfatase|sulfamidase|sulfoglucosamine sulfamidase
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