SFTPB 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SFTPB full-length
Host
Human
Theoretical MW (kDa)
42.02
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SFTPB antibody (H00006439-B01) by Western Blots.
SDS-PAGE Gel
SFTPB transfected lysate.
Western Blot
Lane 1: SFTPB transfected lysate ( 42.02 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SFTPB
Entrez GeneID
6439GeneBank Accession#
NM_000542.2Protein Accession#
NP_000533.2Gene Name
SFTPB
Gene Alias
PSP-B, SFTB3, SFTP3, SMDP1, SP-B
Gene Description
surfactant protein B
Gene Ontology
HyperlinkGene Summary
The SFTPB gene encodes the pulmonary-associated surfactant B protein (SPB), an amphipathic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a lipid-rich material that prevents lung collapse by lowering surface tension at the air-liquid interface in the alveoli of lung. SPB enhances the rate of spreading and increases the stability of surfactant monolayers in vitro. Surfactant is composed of phospholipids and other surfactant-associated proteins (Clark et al., 1995 [PubMed 7644495]). See also SFTPA1 (MIM 178630), SFTPC (MIM 178620), and SFTPD (MIM 178635).[supplied by OMIM
Other Designations
Pulmonary surfactant-associated protein B, 18kD|surfactant, pulmonary-associated protein B
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