SDHB 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SDHB full-length
Host
Human
Theoretical MW (kDa)
31.6
Interspecies Antigen Sequence
Mouse (92); Rat (91)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SDHB antibody (H00006390-D01P) by Western Blots.
SDS-PAGE Gel
SDHB transfected lysate.
Western Blot
Lane 1: SDHB transfected lysate ( 31.60 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SDHB
Entrez GeneID
6390GeneBank Accession#
BC007840.2Protein Accession#
NP_002991.2Gene Name
SDHB
Gene Alias
FLJ92337, IP, PGL4, SDH, SDH1, SDHIP
Gene Description
succinate dehydrogenase complex, subunit B, iron sulfur (Ip)
Gene Ontology
HyperlinkGene Summary
Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq
Other Designations
OTTHUMP00000002396|OTTHUMP00000044624|iron-sulfur subunit
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Citrate cycle (TCA cycle)
- Metabolic pathways
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