S100A12 monoclonal antibody (M01), clone 1F10
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of S100A12 expression in transfected 293T cell line by S100A12 monoclonal antibody (M01), clone 1F10.
Lane 1: S100A12 transfected lysate (Predicted MW: 10.6 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged S100A12 is 1 ng/ml as a capture antibody.
Western Blot detection against Immunogen (35.75 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant S100A12.
Immunogen
S100A12 (NP_005612.1, 1 a.a. ~ 92 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MTKLEEHLEGIVNIFHQYSVRKGHFDTLSKGELKQLLTKELANTIKNIKDKAVIDEIFQGLDANQDEQVDFQEFISLVAIALKAAHYHTHKE
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.75 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of S100A12 expression in transfected 293T cell line by S100A12 monoclonal antibody (M01), clone 1F10.
Lane 1: S100A12 transfected lysate (Predicted MW: 10.6 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged S100A12 is 1 ng/ml as a capture antibody.ELISA
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Gene Info — S100A12
Entrez GeneID
6283GeneBank Accession#
NM_005621.1Protein Accession#
NP_005612.1Gene Name
S100A12
Gene Alias
CAAF1, CAGC, CGRP, ENRAGE, MRP6, p6
Gene Description
S100 calcium binding protein A12
Omim ID
603112Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. [provided by RefSeq
Other Designations
OTTHUMP00000015332|S100 calcium-binding protein A12|S100 calcium-binding protein A12 (calgranulin C)|calgranulin C
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Interactome
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Disease
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