S100A4 monoclonal antibody (M01), clone 1F12-1G7
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More Files
- More Functions
Western Blot (Cell lysate)
S100A4 monoclonal antibody (M01), clone 1F12-1G7 Western Blot analysis of S100A4 expression in Hela ( Cat # L013V1 ).
Western Blot (Cell lysate)
S100A4 monoclonal antibody (M01), clone 1F12-1G7. Western Blot analysis of S100A4 expression in Raw 264.7.
Western Blot (Transfected lysate)
Western Blot analysis of S100A4 expression in transfected 293T cell line by S100A4 monoclonal antibody (M01), clone 1F12-1G7.
Lane 1: S100A4 transfected lysate(11.7 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged S100A4 is approximately 0.1ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to S100A4 on HeLa cell. [antibody concentration 15 ug/ml]
Western Blot detection against Immunogen (36.85 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant S100A4.
Immunogen
S100A4 (AAH16300, 1 a.a. ~ 101 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MACPLEKALDVMVSTFHKYSGKEGDKFKLNKSELKELLTRELPSFLGKRTDEAAFQKLMSNLDSNRDNEVDFQEYCVFLSCIAMMCDEFFEGFPDKQPRKK
Host
Mouse
Reactivity
Human, Mouse
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.85 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
S100A4 monoclonal antibody (M01), clone 1F12-1G7 Western Blot analysis of S100A4 expression in Hela ( Cat # L013V1 ).Western Blot (Cell lysate)
S100A4 monoclonal antibody (M01), clone 1F12-1G7. Western Blot analysis of S100A4 expression in Raw 264.7.Western Blot (Transfected lysate)
Western Blot analysis of S100A4 expression in transfected 293T cell line by S100A4 monoclonal antibody (M01), clone 1F12-1G7.
Lane 1: S100A4 transfected lysate(11.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged S100A4 is approximately 0.1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to S100A4 on HeLa cell. [antibody concentration 15 ug/ml] -
Gene Info — S100A4
Entrez GeneID
6275GeneBank Accession#
BC016300Protein Accession#
AAH16300Gene Name
S100A4
Gene Alias
18A2, 42A, CAPL, FSP1, MTS1, P9KA, PEL98
Gene Description
S100 calcium binding protein A4
Omim ID
114210Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000015467|OTTHUMP00000015468|OTTHUMP00000015469|OTTHUMP00000032895|S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog)|S100 calcium-binding protein A4|S100 calcium-binding protein A4 (calcium prote
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Interactome
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Disease
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Publication Reference
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Endothelial-specific Loss of IFT88 Promotes Endothelial-to-Mesenchymal Transition and Exacerbates Bleomycin-induced Pulmonary Fibrosis.
Singh S, Adam M, Matkar PN, Bugyei-Twum A, Desjardins JF, Chen HH, Nguyen H, Bazinet H, Michels D, Liu Z, Mebrahtu E, Esene L, Joseph J, Ehsan M, Qadura M, Connelly KA, Leong-Poi H, Singh KK.
Scientific Reports 2020 Mar; 10(1):4466.
Application：WB-Tr, Human, Human coronary artery endothelial cells, HUVECs.
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PDGF-mediated mesenchymal transformation renders endothelial resistance to anti-VEGF treatment in glioblastoma.
Liu T, Ma W, Xu H, Huang M, Zhang D, He Z, Zhang L, Brem S, O'Rourke DM, Gong Y, Mou Y, Zhang Z, Fan Y.
Nature Communications 2018 Aug; 9(1):3439.
Application：WB-Ce, WB-Tr, Human, Mouse, Brain endothelial cells, GBM tumor-derived endothelial cells.
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TGFBR-IDH1-Cav1 axis promotes TGF-β signalling in cancer-associated fibroblast.
Hou X, Zhang J, Wang Y, Xiong W, Mi J.
Oncotarget 2017 Sep; 8(48):83962.
Application：WB, Human, Human normal primary fibroblasts.
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Hypoxia-induced changes in the fibroblast secretome, exosome, and whole-cell proteome using cultured, cardiac-derived cells isolated from neonatal mice.
Cosme J, Guo H, Hadipour-Lakmehsari S, Emili A, Gramolini AO.
Journal of Proteome Research 2017 Jul; 16(8):2836.
Application：WB-Ce, Mouse, Mouse cardiac fibroblasts, cardiomyocytes.
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Endothelial-to-Osteoblast Conversion Generates Osteoblastic Metastasis of Prostate Cancer.
Lin SC, Lee YC, Yu G, Cheng CJ, Zhou X, Chu K, Murshed M, Le NT, Baseler L, Abe JI, Fujiwara K, deCrombrugghe B, Logothetis CJ, Gallick GE, Yu-Lee LY, Maity SN, Lin SH.
Developmental Cell 2017 Jun; 41(5):467.
Application：WB-Ce, Mouse, 2H11 cells.
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Cancer-Initiating Cells from Colorectal Cancer Patients Escape from T Cell-Mediated Immunosurveillance In Vitro through Membrane-Bound IL-4.
Volonte A, Di Tomaso T, Spinelli M, Todaro M, Sanvito F, Albarello L, Bissolati M, Ghirardelli L, Orsenigo E, Ferrone S, Doglioni C, Stassi G, Dellabona P, Staudacher C, Parmiani G, Maccalli C.
The Journal of Immunology 2014 Jan; 192(1):523.
Application：IF-CCCC, Human, Cancer-initiating cells from colorectal cancer.
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MiR-21/Smad 7 signaling determines TGF-β1-induced CAF formation.
Li Q, Zhang D, Wang Y, Sun P, Hou X, Larner J, Xiong W, Mi J.
Scientific Reports 2013 Jun; 3:2038.
Application：WB-Ce, WB-Tr, Human, Fibroblast cells.
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Androgen receptor expression in satellite cells of the neonatal levator ani of the rat.
Swift-Gallant A, Monks DA.
Developmental Neurobiology 2013 Jun; 73(6):448.
Application：IF, IHC-Fr, Rat, Rat perinea.
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Identification of novel molecular markers through transcriptomic analysis in human fetal and adult corneal endothelial cells.
Chen Y, Huang K, Nakatsu MN, Xue Z, Deng SX, Fan G.
Human Molecular Genetics 2012 Dec; 22(7):1271.
Application：IF, Human, Adult and fetal cornea tissues.
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Potential role for S100A4 in the disruption of the blood-brain barrier in collagen-induced arthritic mice, an animal model of rheumatoid arthritis.
Nishioku T, Furusho K, Tomita A, Ohishi H, Dohgu S, Shuto H, Yamauchi A, Kataoka Y.
Neuroscience 2011 May; 189:286.
Application：S-ELISA, Mouse, Serum.
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Endothelial-specific Loss of IFT88 Promotes Endothelial-to-Mesenchymal Transition and Exacerbates Bleomycin-induced Pulmonary Fibrosis.
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