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RPS27A 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00006233-T02
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-RPS27A full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 18
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-RPS27A antibody (H00006233-D01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00006233-T02
    RPS27A transfected lysate.
    Western Blot
    QC Testing of H00006233-T02
    Lane 1: RPS27A transfected lysate ( 18.00 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 6233
  • Gene Name:
  • RPS27A
  • Gene Alias:
  • Gene Description:
  • ribosomal protein S27a
  • Gene Summary:
  • Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.[provided by RefSeq
  • Other Designations:
  • 40S ribosomal protein S27a,ubiquitin and ribosomal protein S27a,ubiquitin carboxyl extension protein 80,ubiquitin-CEP80
  • Gene Pathway
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