RPS27 (Human) Recombinant Protein (P01)
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Specification
Product Description
Human RPS27 full-length ORF ( AAH02658, 1 a.a. - 84 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MPLAKDLLHPSPEEEKRKHKKKRLVQSPNSYFMDVKCPGCYKITTVFSHAQTVVLCVGCSTVLCQPTGGKARLTEGCSFRRKQH
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
34.98
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — RPS27
Entrez GeneID
6232GeneBank Accession#
BC002658Protein Accession#
AAH02658Gene Name
RPS27
Gene Alias
MPS-1, MPS1
Gene Description
ribosomal protein S27
Omim ID
603702Gene Ontology
HyperlinkGene Summary
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S27E family of ribosomal proteins. It contains a C4-type zinc finger domain that can bind to zinc. The encoded protein has been shown to be able to bind to nucleic acid. It is located in the cytoplasm as a ribosomal component, but it has also been detected in the nucleus. Studies in rat indicate that ribosomal protein S27 is located near ribosomal protein S18 in the 40S subunit and is covalently linked to translation initiation factor eIF3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq
Other Designations
40S ribosomal protein S27 (metallopan-stimulin 1)|OTTHUMP00000034760|metallopanstimulin 1
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Interactome
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Pathway
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Publication Reference
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A novel whole-cell lysate kinase assay identifies substrates of the p38 MAPK in differentiating myoblasts.
Knight JD, Tian R, Lee RE, Wang F, Beauvais A, Zou H, Megeney LA, Gingras AC, Pawson T, Figeys D, Kothary R.
Skeletal Muscle 2012 Mar; 2:5.
Application:KA, Recombinant protein.
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A novel whole-cell lysate kinase assay identifies substrates of the p38 MAPK in differentiating myoblasts.
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