RPS6KB2 monoclonal antibody (M08), clone 4B11
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant RPS6KB2.
Immunogen
RPS6KB2 (AAH06106, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAAVFDLDLETEEGSEGEGEPELSPADACPLAELRAAGLEPVGHYEEVELTETSVNVGPERIGPHSFELLRVLGKGGYGKVFQVRKVQGTNLGKIYAMKV
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of RPS6KB2 expression in transfected 293T cell line by RPS6KB2 monoclonal antibody (M08), clone 4B11.
Lane 1: RPS6KB2 transfected lysate(53.4 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RPS6KB2 is approximately 0.1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to RPS6KB2 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — RPS6KB2
Entrez GeneID
6199GeneBank Accession#
BC006106Protein Accession#
AAH06106Gene Name
RPS6KB2
Gene Alias
KLS, P70-beta, P70-beta-1, P70-beta-2, S6K-beta2, S6K2, SRK, STK14B, p70(S6K)-beta, p70S6Kb
Gene Description
ribosomal protein S6 kinase, 70kDa, polypeptide 2
Omim ID
608939Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates the S6 ribosomal protein and eucaryotic translation initiation factor 4B (eIF4B). Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation. [provided by RefSeq
Other Designations
S6 kinase-related kinase|p70 ribosomal S6 kinase beta|ribosomal protein S6 kinase, 70kD, polypeptide 2|serine/threonine-protein kinase 14 beta
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Interactome
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Pathway
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Disease
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