RPS6KB1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
RPS6KB1 transfected lysate.
Western Blot
Lane 1: RPS6KB1 transfected lysate ( 57.86 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RPS6KB1 full-length
Host
Human
Theoretical MW (kDa)
57.86
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RPS6KB1 antibody (H00006198-B01) by Western Blots.
SDS-PAGE Gel
RPS6KB1 transfected lysate.
Western Blot
Lane 1: RPS6KB1 transfected lysate ( 57.86 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RPS6KB1
Entrez GeneID
6198GeneBank Accession#
NM_003161Protein Accession#
NP_003152Gene Name
RPS6KB1
Gene Alias
PS6K, S6K, S6K1, STK14A, p70(S6K)-alpha, p70-S6K, p70-alpha
Gene Description
ribosomal protein S6 kinase, 70kDa, polypeptide 1
Omim ID
608938Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized. [provided by RefSeq
Other Designations
p70 S6 kinase, alpha 1|p70 S6 kinase, alpha 2|ribosomal protein S6 kinase, 70kD, polypeptide 1|serine/threonine kinase 14 alpha
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Interactome
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Pathway
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Disease
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