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Last updated: 2017/7/23
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RPS6KB1 monoclonal antibody (M02), clone 1E10

  • Catalog # : H00006198-M02
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against a partial recombinant RPS6KB1.
  • Immunogen:
  • RPS6KB1 (NP_003152, 416 a.a. ~ 525 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
  • Sequence:
  • PSVLESVKEKFSFEPKIRSPRRFIGSPRTPVSPVKFSPGDFWGRGASASTANPQTPVEYPMETSGIEQMDVTMSGEASAPLPIRQPNSGPYKKQAFPMISKRPEHLRMNL
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Isotype:
  • IgG IgA IgM Mix
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.

    QC Testing of H00006198-M02
    Western Blot detection against Immunogen (37.84 KDa) .
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • RPS6KB1 monoclonal antibody (M02), clone 1E10 Western Blot analysis of RPS6KB1 expression in HeLa ( Cat # L013V1 ).
  • PDF DownloadProtocol Download
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Detection limit for recombinant GST tagged RPS6KB1 is approximately 0.03ng/ml as a capture antibody.
  • PDF DownloadProtocol Download
  • ELISA
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • Proximity Ligation Analysis of protein-protein interactions between STK11 and RPS6KB1. HeLa cells were stained with anti-STK11 rabbit purified polyclonal 1:1200 and anti-RPS6KB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • enlarge
  • ELISA
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 6198
  • Gene Name:
  • RPS6KB1
  • Gene Alias:
  • PS6K,S6K,S6K1,STK14A,p70(S6K)-alpha,p70-S6K,p70-alpha
  • Gene Description:
  • ribosomal protein S6 kinase, 70kDa, polypeptide 1
  • Gene Summary:
  • This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized. [provided by RefSeq
  • Other Designations:
  • p70 S6 kinase, alpha 1,p70 S6 kinase, alpha 2,ribosomal protein S6 kinase, 70kD, polypeptide 1,serine/threonine kinase 14 alpha
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