RPN2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
RPN2 transfected lysate.
Western Blot
Lane 1: RPN2 transfected lysate ( 69.52 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RPN2 full-length
Host
Human
Theoretical MW (kDa)
69.52
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RPN2 antibody (H00006185-B01) by Western Blots.
SDS-PAGE Gel
RPN2 transfected lysate.
Western Blot
Lane 1: RPN2 transfected lysate ( 69.52 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RPN2
Entrez GeneID
6185GeneBank Accession#
NM_002951.2Protein Accession#
NP_002942.2Gene Name
RPN2
Gene Alias
RIBIIR, RPN-II, RPNII, SWP1
Gene Description
ribophorin II
Omim ID
180490Gene Ontology
HyperlinkGene Summary
This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit
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