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MRPL12 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00006182-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-MRPL12 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 21.3
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-MRPL12 antibody (H00006182-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00006182-T01
    MRPL12 transfected lysate.
    Western Blot
    QC Testing of H00006182-T01
    Lane 1: MRPL12 transfected lysate ( 21.89 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 6182
  • Gene Name:
  • MRPL12
  • Gene Alias:
  • 5c5-2,FLJ60124,L12mt,MGC8610,MRP-L31/34,MRPL7,MRPL7/L12,RPML12
  • Gene Description:
  • mitochondrial ribosomal protein L12
  • Gene Summary:
  • Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex. [provided by RefSeq
  • Other Designations:
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