RPLP1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RPLP1 full-length
Host
Human
Theoretical MW (kDa)
12.65
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RPLP1 antibody (H00006176-B01) by Western Blots.
SDS-PAGE Gel
RPLP1 transfected lysate.
Western Blot
Lane 1: RPLP1 transfected lysate ( 12.65 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RPLP1
Entrez GeneID
6176GeneBank Accession#
NM_001003.2Protein Accession#
NP_000994.1Gene Name
RPLP1
Gene Alias
FLJ27448, MGC5215, P1, RPP1
Gene Description
ribosomal protein, large, P1
Omim ID
180520Gene Ontology
HyperlinkGene Summary
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal phosphoprotein that is a component of the 60S subunit. The protein, which is a functional equivalent of the E. coli L7/L12 ribosomal protein, belongs to the L12P family of ribosomal proteins. It plays an important role in the elongation step of protein synthesis. Unlike most ribosomal proteins, which are basic, the encoded protein is acidic. Its C-terminal end is nearly identical to the C-terminal ends of the ribosomal phosphoproteins P0 and P2. The P1 protein can interact with P0 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Two alternatively spliced transcript variants that encode different proteins have been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq
Other Designations
60S acidic ribosomal protein P1|acidic ribosomal phosphoprotein P1|ribosomal protein P1
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