RPLP1 monoclonal antibody (M01), clone 1G10
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of RPLP1 expression in transfected 293T cell line by RPLP1 monoclonal antibody (M01), clone 1G10.
Lane 1: RPLP1 transfected lysate (Predicted MW: 11.5 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RPLP1 is 1 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to RPLP1 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (37.9 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant RPLP1.
Immunogen
RPLP1 (NP_000994.1, 1 a.a. ~ 114 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MASVSELACIYSALILHDDEVTVTEDKINALIKAAGVNVEPFWPGLFAKALANVNIGSLICNVGAGGPAPAAGAAPAGGPAPSTAAAPAEEKKVEAKKEESEESDDDMGFGLFD
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.9 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of RPLP1 expression in transfected 293T cell line by RPLP1 monoclonal antibody (M01), clone 1G10.
Lane 1: RPLP1 transfected lysate (Predicted MW: 11.5 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RPLP1 is 1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to RPLP1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — RPLP1
Entrez GeneID
6176GeneBank Accession#
NM_001003.2Protein Accession#
NP_000994.1Gene Name
RPLP1
Gene Alias
FLJ27448, MGC5215, P1, RPP1
Gene Description
ribosomal protein, large, P1
Omim ID
180520Gene Ontology
HyperlinkGene Summary
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal phosphoprotein that is a component of the 60S subunit. The protein, which is a functional equivalent of the E. coli L7/L12 ribosomal protein, belongs to the L12P family of ribosomal proteins. It plays an important role in the elongation step of protein synthesis. Unlike most ribosomal proteins, which are basic, the encoded protein is acidic. Its C-terminal end is nearly identical to the C-terminal ends of the ribosomal phosphoproteins P0 and P2. The P1 protein can interact with P0 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Two alternatively spliced transcript variants that encode different proteins have been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq
Other Designations
60S acidic ribosomal protein P1|acidic ribosomal phosphoprotein P1|ribosomal protein P1
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Interactome
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Pathway
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