RNASE1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
RNASE1 transfected lysate.
Western Blot
Lane 1: RNASE1 transfected lysate ( 17.6 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RNASE1 full-length
Host
Human
Theoretical MW (kDa)
17.6
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RNASE1 antibody (H00006035-B01) by Western Blots.
SDS-PAGE Gel
RNASE1 transfected lysate.
Western Blot
Lane 1: RNASE1 transfected lysate ( 17.6 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RNASE1
Entrez GeneID
6035GeneBank Accession#
NM_002933.3Protein Accession#
NP_002924.1Gene Name
RNASE1
Gene Alias
MGC12408, RIB1, RNS1
Gene Description
ribonuclease, RNase A family, 1 (pancreatic)
Omim ID
180440Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. The encoded protein is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq
Other Designations
RNase upI-1|pancreatic ribonuclease
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Interactome
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Disease
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