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Last updated: 2017/11/19

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RNASE1 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00006035-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-RNASE1 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 17.6
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-RNASE1 antibody (H00006035-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00006035-T01
    RNASE1 transfected lysate.
    Western Blot
    QC Testing of H00006035-T01
    Lane 1: RNASE1 transfected lysate ( 17.6 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 6035
  • Gene Name:
  • RNASE1
  • Gene Alias:
  • MGC12408,RIB1,RNS1
  • Gene Description:
  • ribonuclease, RNase A family, 1 (pancreatic)
  • Gene Summary:
  • This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. The encoded protein is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq
  • Other Designations:
  • RNase upI-1,pancreatic ribonuclease
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