RING1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Western Blot
Lane 1: RING1 transfected lysate ( 41.47 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
RING1 transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RING1 full-length
Host
Human
Theoretical MW (kDa)
41.58
Interspecies Antigen Sequence
Mouse (96); Rat (97)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RING1 antibody (H00006015-B01) by Western Blots.
Western Blot
Lane 1: RING1 transfected lysate ( 41.47 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
RING1 transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RING1
Entrez GeneID
6015GeneBank Accession#
BC002922Protein Accession#
AAH02922Gene Name
RING1
Gene Alias
RING1A, RNF1
Gene Description
ring finger protein 1
Omim ID
602045Gene Ontology
HyperlinkGene Summary
This gene belongs to the RING finger family, members of which encode proteins characterized by a RING domain, a zinc-binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1, EDR1, and CBX4, and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine-rich C-terminal domain. The gene maps to the HLA class II region, where it is contiguous with the RING finger genes FABGL and HKE4. [provided by RefSeq
Other Designations
OTTHUMP00000029416
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Interactome
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Disease
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