RHEB 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
RHEB transfected lysate.
Western Blot
Lane 1: RHEB transfected lysate ( 20.50 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RHEB full-length
Host
Human
Theoretical MW (kDa)
20.5
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RHEB antibody (H00006009-D01P) by Western Blots.
SDS-PAGE Gel
RHEB transfected lysate.
Western Blot
Lane 1: RHEB transfected lysate ( 20.50 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RHEB
Entrez GeneID
6009GeneBank Accession#
NM_005614Protein Accession#
NP_005605.1Gene Name
RHEB
Gene Alias
MGC111559, RHEB2
Gene Description
Ras homolog enriched in brain
Omim ID
601293Gene Ontology
HyperlinkGene Summary
This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. [provided by RefSeq
Other Designations
GTP-binding protein Rheb|Ras homolog enriched in brain 2
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Interactome
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Pathway
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Disease
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