RGS10 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RGS10 full-length
Host
Human
Theoretical MW (kDa)
20.02
Interspecies Antigen Sequence
Mouse (92); Rat (94)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RGS10 antibody (H00006001-B01) by Western Blots.
SDS-PAGE Gel
RGS10 transfected lysate.
Western Blot
Lane 1: RGS10 transfected lysate ( 20.02 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RGS10
Entrez GeneID
6001GeneBank Accession#
NM_001005339.1Protein Accession#
-Gene Name
RGS10
Gene Alias
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Gene Description
regulator of G-protein signaling 10
Omim ID
602856Gene Ontology
HyperlinkGene Summary
Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 10 belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. This protein associates specifically with the activated forms of the two related G-protein subunits, G-alphai3 and G-alphaz but fails to interact with the structurally and functionally distinct G-alpha subunits. Regulator of G protein signaling 10 protein is localized in the nucleus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000020597|OTTHUMP00000069158|regulator of G-protein signalling 10
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Interactome
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