RGS1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Western Blot
Lane 1: RGS1 transfected lysate ( 22.5 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
RGS1 transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RGS1 full-length
Host
Human
Theoretical MW (kDa)
21.67
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RGS1 antibody (H00005996-B02) by Western Blots.
Western Blot
Lane 1: RGS1 transfected lysate ( 22.5 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
RGS1 transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RGS1
Entrez GeneID
5996GeneBank Accession#
NM_002922Protein Accession#
NP_002913Gene Name
RGS1
Gene Alias
1R20, BL34, IER1, IR20
Gene Description
regulator of G-protein signaling 1
Omim ID
600323Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the regulator of G-protein signalling family. This protein is located on the cytosolic side of the plasma membrane and contains a conserved, 120 amino acid motif called the RGS domain. The protein attenuates the signalling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. [provided by RefSeq
Other Designations
B-cell activation protein BL34|OTTHUMP00000033591|early response protein 1R20|immediate-early response 1, B-cell specific|regulator of G-protein signalling 1
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Interactome
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Disease
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