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RFX5 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00005993-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-RFX5 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 67.87
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-RFX5 antibody (H00005993-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00005993-T01
    RFX5 transfected lysate.
    Western Blot
    QC Testing of H00005993-T01
    Lane 1: RFX5 transfected lysate ( 67.87 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 5993
  • Gene Name:
  • RFX5
  • Gene Alias:
  • -
  • Gene Description:
  • regulatory factor X, 5 (influences HLA class II expression)
  • Gene Summary:
  • A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000082795,OTTHUMP00000196318,regulatory factor X, 5
  • Related Disease
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