RFX3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
RFX3 transfected lysate.
Western Blot
Lane 1: RFX3 transfected lysate ( 82.5 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RFX3 full-length
Host
Human
Theoretical MW (kDa)
82.5
Interspecies Antigen Sequence
Mouse (99)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RFX3 antibody (H00005991-B01) by Western Blots.
SDS-PAGE Gel
RFX3 transfected lysate.
Western Blot
Lane 1: RFX3 transfected lysate ( 82.5 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RFX3
Entrez GeneID
5991GeneBank Accession#
NM_134428.1Protein Accession#
-Gene Name
RFX3
Gene Alias
MGC87155, bA32F11.1
Gene Description
regulatory factor X, 3 (influences HLA class II expression)
Omim ID
601337Gene Ontology
HyperlinkGene Summary
This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X2, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. Two transcript variants encoding different isoforms have been described for this gene, and at least one of the variants utilizes alternative polyadenylation signals. [provided by RefSeq
Other Designations
DNA binding protein RFX3|OTTHUMP00000020993|OTTHUMP00000020994|OTTHUMP00000044314|regulatory factor X3
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