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RFC5 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00005985-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-RFC5 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 38.5
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-RFC5 antibody (H00005985-B02) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00005985-T01
    RFC5 transfected lysate.
    Western Blot
    QC Testing of H00005985-T01
    Lane 1: RFC5 transfected lysate ( 38.50 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 5985
  • Gene Name:
  • RFC5
  • Gene Alias:
  • MGC1155,RFC36
  • Gene Description:
  • replication factor C (activator 1) 5, 36.5kDa
  • Gene Summary:
  • The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
  • Other Designations:
  • A1 36 kDa subunit,RFC, 36.5 kD subunit,activator 1 36 kDa subunit,replication factor C (activator 1) 5 (36.5kD),replication factor C 5
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