RFC5 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
RFC5 transfected lysate.
Western Blot
Lane 1: RFC5 transfected lysate ( 38.50 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RFC5 full-length
Host
Human
Theoretical MW (kDa)
38.5
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RFC5 antibody (H00005985-B02) by Western Blots.
SDS-PAGE Gel
RFC5 transfected lysate.
Western Blot
Lane 1: RFC5 transfected lysate ( 38.50 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RFC5
Entrez GeneID
5985GeneBank Accession#
NM_007370Protein Accession#
NP_031396Gene Name
RFC5
Gene Alias
MGC1155, RFC36
Gene Description
replication factor C (activator 1) 5, 36.5kDa
Omim ID
600407Gene Ontology
HyperlinkGene Summary
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
A1 36 kDa subunit|RFC, 36.5 kD subunit|activator 1 36 kDa subunit|replication factor C (activator 1) 5 (36.5kD)|replication factor C 5
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Interactome
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Pathway
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Disease
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